Large-scale preparation, purification, and characterization of hyaluronan oligosaccharides from 4-mers to 52-mers.
Identifieur interne : 000146 ( Ncbi/Merge ); précédent : 000145; suivant : 000147Large-scale preparation, purification, and characterization of hyaluronan oligosaccharides from 4-mers to 52-mers.
Auteurs : Akira Tawada [Japon] ; Takahiro Masa ; Yoji Oonuki ; Atsushi Watanabe ; Yuji Matsuzaki ; Akira AsariSource :
- Glycobiology [ 0959-6658 ] ; 2002.
Descripteurs français
- KwdFr :
- Acide hyaluronique (), Acide hyaluronique (isolement et purification), Acide hyaluronique (synthèse chimique), Chromatographie en phase liquide à haute performance, Oligosaccharides (), Oligosaccharides (isolement et purification), Oligosaccharides (synthèse chimique), Spectrométrie de masse ESI, Électrophorèse ().
- MESH :
- isolement et purification : Acide hyaluronique, Oligosaccharides.
- synthèse chimique : Acide hyaluronique, Oligosaccharides.
- Acide hyaluronique, Chromatographie en phase liquide à haute performance, Oligosaccharides, Spectrométrie de masse ESI, Électrophorèse.
English descriptors
- KwdEn :
- Chromatography, High Pressure Liquid, Electrophoresis (methods), Hyaluronic Acid (chemical synthesis), Hyaluronic Acid (chemistry), Hyaluronic Acid (isolation & purification), Oligosaccharides (chemical synthesis), Oligosaccharides (chemistry), Oligosaccharides (isolation & purification), Spectrometry, Mass, Electrospray Ionization.
- MESH :
- chemical , chemical synthesis : Hyaluronic Acid, Oligosaccharides.
- chemical , chemistry : Hyaluronic Acid, Oligosaccharides.
- chemical , isolation & purification : Hyaluronic Acid, Oligosaccharides.
- methods : Electrophoresis.
- Chromatography, High Pressure Liquid, Spectrometry, Mass, Electrospray Ionization.
Abstract
Hyaluronan (HA) was depolymerized by partial digestion with testicular hyaluronidase and separated into size-uniform HA oligosaccharides from 4-mers to 52-mers by anion exchange chromatography after removal of the hyaluronidase. The purity and size of each HA oligosaccharide was confirmed by using HPLC analyses, FACE, and ESI-MS. (1)H and (13)C NMR assignments and elemental analyses were obtained for each HA oligosaccharide. Endotoxins, proteins, and DNA were absent or in trace amounts in these HA oligosaccharides. Gram/mg-scale hyaluronan oligosaccharides were obtained from 200 g of HA starting material. These pure, size-uniform, and large range of HA oligosaccharides will be available for investigating important biological functions of HA, such as for the determination of the size(s) of HA oligosaccharides that induce angiogenesis or mediate inflammatory responses, and to interact with HA-binding proteins and receptors both in in vitro and in vivo studies.
DOI: 10.1093/glycob/cwf048
PubMed: 12122023
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Yoji" uniqKey="Oonuki Y" first="Yoji" last="Oonuki">Yoji Oonuki</name></author><author><name sortKey="Watanabe, Atsushi" sort="Watanabe, Atsushi" uniqKey="Watanabe A" first="Atsushi" last="Watanabe">Atsushi Watanabe</name></author><author><name sortKey="Matsuzaki, Yuji" sort="Matsuzaki, Yuji" uniqKey="Matsuzaki Y" first="Yuji" last="Matsuzaki">Yuji Matsuzaki</name></author><author><name sortKey="Asari, Akira" sort="Asari, Akira" uniqKey="Asari A" first="Akira" last="Asari">Akira Asari</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2002">2002</date><idno type="RBID">pubmed:12122023</idno><idno type="pmid">12122023</idno><idno type="doi">10.1093/glycob/cwf048</idno><idno type="wicri:Area/PubMed/Corpus">002500</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002500</idno><idno type="wicri:Area/PubMed/Curation">002500</idno><idno type="wicri:explorRef" wicri:stream="PubMed" 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xml:lang="en"><term>Hyaluronic Acid</term><term>Oligosaccharides</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Hyaluronic Acid</term><term>Oligosaccharides</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Hyaluronic Acid</term><term>Oligosaccharides</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Acide hyaluronique</term><term>Oligosaccharides</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Electrophoresis</term></keywords><keywords scheme="MESH" qualifier="synthèse chimique" xml:lang="fr"><term>Acide hyaluronique</term><term>Oligosaccharides</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Chromatography, High Pressure Liquid</term><term>Spectrometry, Mass, Electrospray Ionization</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Acide 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The purity and size of each HA oligosaccharide was confirmed by using HPLC analyses, FACE, and ESI-MS. (1)H and (13)C NMR assignments and elemental analyses were obtained for each HA oligosaccharide. Endotoxins, proteins, and DNA were absent or in trace amounts in these HA oligosaccharides. Gram/mg-scale hyaluronan oligosaccharides were obtained from 200 g of HA starting material. These pure, size-uniform, and large range of HA oligosaccharides will be available for investigating important biological functions of HA, such as for the determination of the size(s) of HA oligosaccharides that induce angiogenesis or mediate inflammatory responses, and to interact with HA-binding proteins and receptors both in in vitro and in vivo studies.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">12122023</PMID><DateCompleted><Year>2003</Year><Month>01</Month><Day>22</Day></DateCompleted><DateRevised><Year>2019</Year><Month>05</Month><Day>13</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0959-6658</ISSN><JournalIssue CitedMedium="Print"><Volume>12</Volume><Issue>7</Issue><PubDate><Year>2002</Year><Month>Jul</Month></PubDate></JournalIssue><Title>Glycobiology</Title><ISOAbbreviation>Glycobiology</ISOAbbreviation></Journal><ArticleTitle>Large-scale preparation, purification, and characterization of hyaluronan oligosaccharides from 4-mers to 52-mers.</ArticleTitle><Pagination><MedlinePgn>421-6</MedlinePgn></Pagination><Abstract><AbstractText>Hyaluronan (HA) was depolymerized by partial digestion with testicular hyaluronidase and separated into size-uniform HA oligosaccharides from 4-mers to 52-mers by anion exchange chromatography after removal of the hyaluronidase. The purity and size of each HA oligosaccharide was confirmed by using HPLC analyses, FACE, and ESI-MS. (1)H and (13)C NMR assignments and elemental analyses were obtained for each HA oligosaccharide. Endotoxins, proteins, and DNA were absent or in trace amounts in these HA oligosaccharides. Gram/mg-scale hyaluronan oligosaccharides were obtained from 200 g of HA starting material. These pure, size-uniform, and large range of HA oligosaccharides will be available for investigating important biological functions of HA, such as for the determination of the size(s) of HA oligosaccharides that induce angiogenesis or mediate inflammatory responses, and to interact with HA-binding proteins and receptors both in in vitro and in vivo studies.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Tawada</LastName><ForeName>Akira</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Seikagaku Corporation, Central Research Laboratories, 1253, Tateno 3-chome, Higshiyamato-shi, Tokyo 207-0021, Japan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Masa</LastName><ForeName>Takahiro</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Oonuki</LastName><ForeName>Yoji</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Watanabe</LastName><ForeName>Atsushi</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Matsuzaki</LastName><ForeName>Yuji</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Asari</LastName><ForeName>Akira</ForeName><Initials>A</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>Glycobiology</MedlineTA><NlmUniqueID>9104124</NlmUniqueID><ISSNLinking>0959-6658</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D009844">Oligosaccharides</NameOfSubstance></Chemical><Chemical><RegistryNumber>9004-61-9</RegistryNumber><NameOfSubstance UI="D006820">Hyaluronic Acid</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002851" MajorTopicYN="N">Chromatography, High Pressure Liquid</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004586" MajorTopicYN="N">Electrophoresis</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006820" MajorTopicYN="N">Hyaluronic Acid</DescriptorName><QualifierName UI="Q000138" MajorTopicYN="N">chemical synthesis</QualifierName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D009844" MajorTopicYN="N">Oligosaccharides</DescriptorName><QualifierName UI="Q000138" MajorTopicYN="N">chemical synthesis</QualifierName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D021241" MajorTopicYN="N">Spectrometry, Mass, Electrospray Ionization</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2002</Year><Month>7</Month><Day>18</Day><Hour>10</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2003</Year><Month>1</Month><Day>23</Day><Hour>4</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2002</Year><Month>7</Month><Day>18</Day><Hour>10</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">12122023</ArticleId><ArticleId IdType="doi">10.1093/glycob/cwf048</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Japon</li></country><region><li>Région de Kantō</li></region><settlement><li>Tokyo</li></settlement></list><tree><noCountry><name sortKey="Asari, Akira" sort="Asari, Akira" uniqKey="Asari A" first="Akira" last="Asari">Akira Asari</name><name sortKey="Masa, Takahiro" sort="Masa, Takahiro" uniqKey="Masa T" first="Takahiro" last="Masa">Takahiro Masa</name><name sortKey="Matsuzaki, Yuji" sort="Matsuzaki, Yuji" uniqKey="Matsuzaki Y" first="Yuji" last="Matsuzaki">Yuji Matsuzaki</name><name sortKey="Oonuki, Yoji" sort="Oonuki, Yoji" uniqKey="Oonuki Y" first="Yoji" last="Oonuki">Yoji Oonuki</name><name sortKey="Watanabe, Atsushi" sort="Watanabe, Atsushi" uniqKey="Watanabe A" first="Atsushi" last="Watanabe">Atsushi Watanabe</name></noCountry><country name="Japon"><region name="Région de Kantō"><name sortKey="Tawada, Akira" sort="Tawada, Akira" uniqKey="Tawada A" first="Akira" last="Tawada">Akira Tawada</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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